egfr family members  (Santa Cruz Biotechnology)

Journal: Breast Cancer Research : BCR

Article Title: Simultaneous targeting of HER family pro-survival signaling with Pan-HER antibody mixture is highly effective in TNBC: a preclinical trial with PDXs

doi: 10.1186/s13058-020-01280-z

Figure Lengend Snippet: Analysis of HER family members and associated signaling pathways. Untreated PDX tumor models were analyzed by Western blot to determine the basal, initial levels of expression of HER family members EGFR and HER3, as well as associated signaling pathways including pEGFR, pHER3, PTEN, and FAK. A representative image is shown

Article Snippet: Pan-HER, a mixture of six monoclonal antibodies directed against each of the HER family members EGFR, HER2, and HER3, was generously provided by Symphogen A/S (Denmark) [ , , ].

Techniques: Western Blot, Expressing

Journal: Breast Cancer Research : BCR

Article Title: Simultaneous targeting of HER family pro-survival signaling with Pan-HER antibody mixture is highly effective in TNBC: a preclinical trial with PDXs

doi: 10.1186/s13058-020-01280-z

Figure Lengend Snippet: Analysis of HER family members and associated signaling pathways in representative vehicle- and Pan-HER-treated TNBC PDXs. a Representative PDX tumor models BCM-2665, BCM-3107 (subgroup 2), BCM-4913, and BMC-3555 (subgroup 1) treated with either vehicle control or Pan-HER for 3 cycles were collected at the end of the experiment (day 21 following the initial injection) and analyzed by Western blot ( a ) or immunohistochemistry ( b ). Western blot analysis of tumor lysates was used to determine HER family members EGFR and HER3, as well as associated signaling pathways including pEGFR, pHER3, FAK, AKT, and ERK pathways. A representative image is shown. β-actin levels were used as loading controls. Each Western blot lane corresponds to an individual PDX/mouse of the designated group. b Representative immunohistochemistry analysis of human EGFR and HER3 protein expression was performed in preparations of TNBC tumor samples as described in “ ”; samples were collected as described above. Counterstain: hematoxylin; here where it says "magnification x 4", it should say "magnification x 20".

Article Snippet: Pan-HER, a mixture of six monoclonal antibodies directed against each of the HER family members EGFR, HER2, and HER3, was generously provided by Symphogen A/S (Denmark) [ , , ].

Techniques: Injection, Western Blot, Immunohistochemistry, Expressing